Composite

Part:BBa_K4156112:Design

Designed by: Zheng Huang   Group: iGEM22_LZU-CHINA   (2022-10-07)


plac-LuxCDABE


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 328
    Illegal BglII site found at 2309
    Illegal BglII site found at 5561
    Illegal BamHI site found at 872
    Illegal BamHI site found at 5861
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 741
    Illegal NgoMIV site found at 1368
    Illegal AgeI site found at 5879
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

plac-LuxCDABE is a complex regulatory system of the plac operon ( BBa_R0010 ) and the LuxCDABE operon.


Source

Experimental constructed.

References